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目的:研究垂体瘤转化基因在非小细胞肺癌组织、肺良性病变组织和正常支气管黏膜上皮组织中的表达,初步探讨其与非小细胞肺癌发生发展,侵袭转移的关系。方法:(1)用SP免疫组化法检测76例临床石蜡组织标本(44例非小细胞肺癌、20例肺良性病变组织和12例正常支气管黏膜上皮组织)中的PTTG蛋白的表达。(2)用RTPCR法分析PTTG mRNA在不同性质肺组织中的表达情况。结果:(1)PTTG蛋白在不同性质肺组织中的表达差别具有明显差异;在TNM分期、淋巴结转移组间差别有统计学意义。(2)PTTG mRNA在不同性质肺组织中的表达差别具有明显差异(P<0.001);在TNM分期、淋巴结转移组间差别有统计学意义。结论:细胞肺癌的发生发展及转移有关。
Objective: To study the expression of pituitary tumor transforming gene in non-small cell lung cancer, benign lung disease and normal bronchial mucosa epithelium, and to explore its relationship with the development, invasion and metastasis of non-small cell lung cancer. Methods: (1) The expression of PTTG protein in 76 cases of paraffin-embedded tissues (44 cases of non-small cell lung cancer, 20 cases of lung benign lesions and 12 cases of normal bronchial mucosa epithelial tissues) was detected by SP immunohistochemistry. (2) The expression of PTTG mRNA in different types of lung tissue was analyzed by RTPCR. Results: (1) There was significant difference in the expression of PTTG protein among different types of lung tissues. There was significant difference between TNM staging and lymph node metastasis group. (2) There was a significant difference in the expression of PTTG mRNA in different types of lung tissues (P <0.001). There was a significant difference between the two groups in TNM stage and lymph node metastasis. Conclusion: The occurrence and development of lung cancer and metastasis.