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对立枯丝核菌(Rhizoctonia solani Kühn)和禾谷丝核菌(R.cerealis Vander Hoeven)的16个菌丝融合群或亚群的标准菌株及来自江苏大麦纹枯病的9个菌丝融合群或亚群共68个菌株进行了聚丙烯酰胺凝胶电泳,测定酯酶同工酶。结果表明:1.立枯丝核菌主酶带数的变幅范围比禾谷丝核菌大;2.无论禾谷丝核菌,还是立枯丝核菌,各菌丝融合群或亚群之间的电泳图谱都有显著差异,但与各自对应的融合群或亚群的标准菌株的图谱则相似;3.44个大麦禾谷丝核菌CAG-1群菌株间的图谱也有差异,但都有一条共同的主酶带(E_4);4.据主副酶带数目和位置,将禾谷丝核菌CAG-1群菌株再分为2个类型(Ⅰ型和Ⅱ型)和5个亚型(Ⅰa、Ⅰb、Ⅰc和Ⅱa、Ⅱb);酶谱类型与菌株的采集品种和致病性无关,但与采集地点似有一定的联系。
Rhizoctonia solani Kühn and R.cerealis Vander Hoeven 16 mycelium fusion group or subgroup of standard strains and from Jiangsu barley blight 9 mycelial fusion group Or subgroup of 68 strains of polyacrylamide gel electrophoresis, determination of esterase isoenzyme. The results showed that: 1 Rhizoctonia solani main enzyme band amplitude range greater than Rhizoctonia cereali; 2 regardless of the Rhizoctonia cerealis, or Rhizoctonia solani, mycelium fusion group or subgroup There were significant differences in the electrophoretograms between the two groups, but the maps of the corresponding isolates or subgroups were similar. 3. There were also differences in the patterns of C44 isolates among the 44 strains of H. armigera, but both A common major enzyme band (E_4); 4. According to the number and location of the major and minor enzyme bands, the Rhizoctonia cerealis CAG-1 group was subdivided into two types (type I and type II) and five subtypes (Ⅰa, Ⅰb, Ⅰc and Ⅱa, Ⅱb). The types of zymogram were not related to the pathogenicity of the strains, but they were related to the collection sites.