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目的:观察加味青娥丸对激素性股骨头坏死小鼠局部1,25(OH)_2D_3/VDR mRNA/RAS信号转导通路的影响,以探讨加味青娥丸治疗激素性股骨头坏死的机制。方法:8周龄SPF级C57BL/6雄性小鼠随机分5组,每组12只,即空白对照组(A组)、激素性骨坏死模型组(B组)、青娥丸加味组(C组)、骨化三醇干预组(D组)及血管紧张素转换酶抑制剂(ACEI)干预组(E组),治疗半年后,运用micro-CT检测并经三维重建获得股骨头颈局部骨组织微观结构,检测股骨头颈局部1,25(OH)_2D_3/VDR mRNA/RAS系统(ACE,AngⅡmRNA)表达情况。结果:空白对照组(A组)骨体积分数(BV/TV)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)较模型组(B组)显著增加,差异有统计学意义(P<0.05),结构模型指数(SMI)、骨小梁间隙(Tb.Sp)、骨表面积体积比(BS/BV)较模型组(B组)明显降低,差异有统计学意义(P<0.05),模型组股骨头局部骨小梁稀疏,孔隙率增高,结构紊乱,部分骨小梁结构不完整,表明模型成功。治疗后C组、D组及E组1,25(OH)_2D_3,VDR mRNA表达均较模型组高,差异有统计学意义(P<0.05),而ACE及AngⅡmRNA表达较模型组低,差异有统计学意义(P<0.05)。结论:加味青娥丸可能通过调节1,25(OH)_2D_3/VDR mRNA/RAS信号转导通路而对股骨头坏死疾病的进展起到一定的治疗作用。
Objective: To observe the effect of Jiawei Qing’e pill on local 1,25 (OH) _2D_3 / VDR mRNA / RAS signal transduction pathway in steroid-induced osteonecrosis of the femoral head in order to investigate the mechanism of Jiawei Qing’e pill in treating steroid-induced osteonecrosis of the femoral head. Methods: 8-week-old male SPF C57BL / 6 mice were randomly divided into 5 groups (n = 12 each), namely blank control group (group A), osteoporosis model group (group B) (Group D) and ACEI intervention group (group E). After six months of treatment, the bone tissue of the femoral head and neck was obtained by micro-CT examination and three-dimensional reconstruction Microstructure, the detection of femoral head and neck 1,25 (OH) _2D_3 / VDR mRNA / RAS system (ACE, Ang Ⅱ mRNA) expression. Results: The BV / TV, Tb.N, Tb.Th of blank control group (A group) were significantly higher than those of model group (B group) (P <0.05). The SMI, Tb.Sp and BS / BV of the model group were significantly lower than those of the model group (P <0.05), and the difference was statistically significant (P < P <0.05). In the model group, the local trabecular bone became thinner and the porosity increased. The structure was disorganized and some trabecular structures were incomplete, indicating that the model was successful. After treatment, the expressions of 1,25 (OH) _2D_3 and VDR mRNA in group C, group D and group E were significantly higher than that in model group (P <0.05), while the expression of ACE and AngⅡ mRNA in model group was lower than that in model group Statistical significance (P <0.05). Conclusion: Jiawei Qing’e pills may play a therapeutic role in the progression of femoral head necrosis by regulating 1,25 (OH) _2D_3 / VDR mRNA / RAS signal transduction pathway.