论文部分内容阅读
用生物学方法检测28例慢活肝患者PBMC的IL-2活性,用葡萄球菌免疫球蛋白间接花环法检测其IL-2R表达细胞(IL-2R~+,mIL-2R)的百分率,用ELISA法定sIL-2R。结果显示慢活肝患者IL-2与IL-2R~+均较正常对照值明显下降(IL-2分别为99.04±26.22与184.15±84.09,P<0.01;IL-2R~+分别为37.15±12.15与54.76±8.91,P<0.01;IL-2R~+分别为37.15±12.15与54.76±8.91,P<0.01),且两者下降呈正相关(n=28,r=0.705,P<0.01)。与此同时,慢活肝患者sIL-2R较正常对照值明显增高(sIL-2R分别为314.6±199.2与65±31.5,P<0.05),sIL-2R的增高与ALT的升高相关(ALT异常组与正常的sIL-2R分别为349.23±199.75与139.0±48.48,P<0.05)。提示慢活肝患者存在免疫调节功能异常,sIL-2R在免疫调节中具有重要意义。
IL-2 activity in peripheral blood mononuclear cells (PBMCs) from 28 patients with chronic liver disease was detected by the biological method. The percentage of IL-2R-expressing cells (IL-2R ~ +, mIL-2R) was detected by staphylococcal indirect immunocytochemistry. Legal sIL-2R. The results showed that the levels of IL-2 and IL-2R ~ + in IL-2 and IL-2R ~ + were significantly decreased in patients with chronic liver disease (IL-2 were 99.04 ± 26.22 and 184.15 ± 84.09 respectively, P <0.01; IL-2R ~ + were 37.15 ± 12.15 (54.76 ± 8.91, P <0.01; IL-2R ~ + were 37.15 ± 12.15 and 54.76 ± 8.91, P <0.01 respectively), and there was a positive correlation between them (n = 28, r = 0.705, P <0.01). At the same time, sIL-2R was significantly higher in patients with chronic liver disease than in normal controls (sIL-2R = 314.6 ± 199.2 vs 65 ± 31.5, P <0.05) and increased sIL-2R was associated with increased ALT Group and normal sIL-2R were 349.23 ± 199.75 and 139.0 ± 48.48, P <0.05). Prompt immune liver function in patients with immune dysfunction, sIL-2R in immune regulation is of great significance.