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以171个F2双核体菌株为作图群体,通过相互配对的2个单核体的基因型推断双核体基因型,构建了第一张基于双核体群体的香菇遗传图谱。该图谱包含分布于15个连锁群的459个标记,覆盖长度为989.7cM,平均标记间隔为2.2cM。此外,以此双核体群体作为表型分离群体,定位了6个与香菇双核体菌丝生长速度相关的QTLs,位于5个连锁群上。采用全同胞单核体随机交配策略,易于构建相对大的双核体群体,用于连锁图构建和QTL定位。研究表明,在食用菌连锁图谱构建及QTL定位研究中,利用F2群体,可能为提高遗传作图效率,解决作图群体与表型分离群体间不一致问题提供新的途径。
A total of 171 F2 dinuclear isolates were used as the mapping population. The dinucleus genotypes were deduced from the two haplotypes paired with each other to construct the first genetic map based on the dinuclear population. The map contains 459 markers distributed over 15 linkage groups, covering a length of 989.7 cM with an average marker interval of 2.2 cM. In addition, using the dinuclear population as a phenotypic segregation population, six QTLs related to the mycelial growth rate of the dinofungi were located and located on five linkage groups. Using the all-sibling monokaryotic mating strategy, it is easy to construct a relatively large dinuclear population for linkage map construction and QTL mapping. The research shows that in the edible fungus linkage map construction and QTL mapping, the use of F2 population may provide a new way to improve the efficiency of genetic mapping and solve the inconsistency between the mapping population and phenotypic isolation population.