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本文研究了口服L-精氨酸对结直肠癌患者肿瘤组织内浸润性淋巴细胞(TILs)的调节作用,从而了解其是否能增强NK和LAK活性细胞对瘤体微环境的浸润作用。选择18例结直肠癌患者,肝肾功能正常,实验前4周内均未接受过化疗、放疗或免疫治疗。随机分为对照组(标准饮食组,蛋白摄入60g/d,含精氨酸3.2g)和实验组(术前3天饮食补充L-精氨酸30g/d),术前晚22:00后禁食,次日肿瘤切除,术中切取活组织置入液氮中快速冷冻,-70℃保存待免疫染色用,其余肿瘤组织固定在中性甲醛液中。标本采用碱性磷酸酶-抗碱性磷酸酶染色显示TILs免疫表型,标记单抗试剂分别为CD2(T细胞)、CD3(T细胞)、CD4(Th细胞)、CD8(Ts细胞)、CD16(NK细胞)、CDl9(B细胞)、CD56(NK、LAK细胞、单核细胞)和CD68(单核、
In this study, we investigated the effect of oral L-arginine on the regulation of infiltrating lymphocytes (TILs) in colorectal cancer patients to determine whether they could enhance the infiltration of NK and LAK-active cells into the tumor microenvironment. 18 patients with colorectal cancer were selected and their liver and kidney function were normal. No chemotherapy, radiotherapy or immunotherapy was performed within 4 weeks before the experiment. Randomly divided into control group (standard diet group, protein intake 60g/d, containing arginine 3.2g) and experimental group (3 days before surgery diet supplement L-arginine 30g/d), before surgery 22:00 After fasting, the tumor was excised the following day. Live tissue was harvested and placed in liquid nitrogen for rapid freezing. The samples were stored at -70°C until immunostaining, and the remaining tumor tissues were fixed in neutral formaldehyde. The specimens were stained with alkaline phosphatase-anti-alkaline phosphatase to show the TILs immunophenotype. The labeled monoclonal antibodies were CD2 (T cells), CD3 (T cells), CD4 (Th cells), CD8 (Ts cells), and CD16. (NK cells), CDl9 (B cells), CD56 (NK, LAK cells, monocytes) and CD68 (mononuclear,