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用免疫组织化学ＡＢＣ法，观察２５例涎腺腺样囊性癌（ＡＣＣ）患者的癌组织中Ⅳ型胶原以及Ⅳ型胶原酶的表达与分布，探讨其与ＡＣＣ侵袭和转移的关系。结果显示，Ⅳ型胶原主要分布在基底膜上以及部分筛状假囊、腺管和肿瘤细胞浆内，Ⅳ型胶原酶分布在肿瘤细胞浆内及周围。经统计学检验，发现筛状－管状型ＡＣＣ，ＴＮＭ早期和无转移者，Ⅳ型胶原多阳性表达，Ⅳ型胶原酶多阴性表达；而实体型ＡＣＣ，ＴＮＭ晚期和转移者，Ⅳ型胶原多阴性表达，Ⅳ型胶原酶多阳性表达。本研究结果表明，Ⅳ型胶原低表达和Ⅳ型胶原酶的高表达可作为判定ＡＣＣ临床恶性程度的指征，是ＡＣＣ侵袭和转移的关键因素之一。
Using immunohistochemical ABC method, the expression and distribution of type IV collagen and type IV collagenase in cancerous tissues of 25 patients with adenoid cystic carcinoma of the salivary gland (ACC) were observed, and their relationship with invasion and metastasis of ACC was explored. The results showed that type IV collagen was mainly distributed on the basement membrane and part of the cystic pseudocapsules, ducts, and tumor cytoplasm. Type IV collagenase was distributed in and around the cytoplasm of tumors. After statistical tests, it was found that there was more positive expression of type IV collagen and more negative expression of type IV collagenase in sieve-tubular type ACC, early and non-metastasis TNM, and more negative expression of type IV collagenase; Negative expression, type IV collagenase positive expression. The results of this study indicate that the low expression of type IV collagen and the high expression of type IV collagenase can be used as an indication for the clinical malignancy of ACC, and it is one of the key factors for the invasion and metastasis of ACC.