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为研究ＶＰ－１６对雄激素非依赖性前列腺癌细胞ＰＣ－３凋亡的诱导作用，应用ＨＥ染色观察细胞的形态学改变，以ＤＮＡ凝胶电泳和流式细胞术（ＦＣＭ）分析细胞凋亡和细胞周期分布，同时应用３Ｈ－ＴｄＲ掺入法研究ＶＰ－１６对ＰＣ－３细胞ＤＮＡ合成的影响。结果表明，（１）凋亡的ＰＣ－３细胞呈明显的形态学改变；（２）ＤＮＡ断裂呈梯形变化；（３）ＰＣ－３细胞凋亡率随ＶＰ－１６作用浓度增加和时间延长逐渐增高；（４）ＶＰ－１６能够明显抑制ＰＣ－３细胞ＤＮＡ的合成。提示ＶＰ－１６可诱导前列腺肿瘤细胞凋亡。
To investigate the effect of VP-16 on the induction of apoptosis in androgen-independent prostate cancer cell line PC-3, the morphological changes of the cells were observed by HE staining. The apoptosis was analyzed by DNA gel electrophoresis and flow cytometry (FCM) And cell cycle distribution, and 3H-TdR incorporation assay was used to study the effect of VP-16 on DNA synthesis in PC-3 cells. The results showed that: (1) apoptotic PC-3 cells showed obvious morphological changes; (2) DNA breaks showed a trapezoidal change; (3) The apoptosis rate of PC-3 cells gradually increased with the increase of VP- (4) VP-16 can significantly inhibit the DNA synthesis of PC-3 cells. Tip VP-16 can induce prostate tumor cell apoptosis.