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目的迟缓爱德华氏菌是目前水产养殖中主要的病原菌,其感染谱甚广,危害巨大。因此,研制能有效预防这种致病菌的疫苗,将会极大的促进水产养殖业的发展。方法用OMP-LPS偶联物、OMP、LPS、FKC(福尔马林灭活全菌)以及生理盐水对照组按相同程序分别免疫鳗鲡。通过溶菌酶活性检测、微量凝集反应试验,结果鳗鲡经OMP-LPS偶联物、OMP、LPS以及FKC疫苗免疫后,各免疫组间血清中溶菌酶活性没有显著差异(P>0.05),但均远高于生理盐水对照组(P<0.05);OMP-LPS偶联组抗迟缓爱德华氏菌(E.tarda)E29L株的抗体效价较单纯的LPS组、OMP组,FKC组抗体滴度高,且持续时间长;对迟缓爱德华氏菌(E.tarda)E29L株的攻击,OMP-LPS偶联组保护率为84%,高于单纯OMP组的80%,LPS组的44%和FKC组60%。结论 OMP-LPS偶联物、OMP、LPS、FKC添加佐剂ISA763,各组的溶菌酶活性均显著高于不添加佐剂各组(P<0.05),除了FKC+ISA763佐剂组外,其余各组均与不添加佐剂各组差异极显著(P<0.01),且添加佐剂各组保护率都明显高于不添加佐剂各组,说明添加ISA763佐剂能够更好的启动鱼类的特异性和非特异性免疫。
The purpose of retarding Edwardsiella is currently the major pathogen in aquaculture, its spectrum of infection is very wide, a great harm. Therefore, the development of a vaccine that can effectively prevent this pathogen will greatly promote the development of aquaculture. Methods EMP was immunized with OMP-LPS conjugate, OMP, LPS, FKC (formalin-inactivated whole bacteria) and normal saline control group by the same procedure. The result of lysozyme activity and microagglutination test showed that there was no significant difference in the activity of lysozyme between immunized groups (P> 0.05) after immunization with OMP-LPS conjugate, OMP, LPS and FKC vaccine (P <0.05). The antibody titer of E.tarda strain E29L in OMP-LPS conjugate group was higher than that in LPS group, OMP group and FKC group , And lasted for a long time. For E. tarda strain E29L, the protection rate of OMP-LPS conjugate group was 84%, higher than 80% of OMP group, 44% of LPS group and FKC group 60%. Conclusion The addition of adjuvant ISA763 to OMP-LPS conjugate, OMP, LPS and FKC significantly increased the lysozyme activity of all groups (P <0.05). Except FKC + ISA763 adjuvant group, the rest There was significant difference (P <0.01) among each group with or without adjuvant, and the protective rates in each group were significantly higher than those without adjuvant, indicating that the addition of ISA763 adjuvant can better start the fish Specific and non-specific immunity.