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目的： 建立霉酚酸（MPA）及其葡糖苷酸（MPAG）血药浓度测定方法。方法：采用高效液相色谱（HPLC）－二极管阵列检测器测定血药浓度，检测波长 254nm。血样加乙腈沉淀蛋白。色谱柱：Hypersil ODS2（200 min×4.6 mm，5 μm）；流动相:A相为25％CH3CN＋75％0．02 mol／L KH2PO4．（pH 3．0）,B相为 70％CH3CN＋30％0．02 mol／L K2HPO4（pH6．5），梯度洗脱；流速：1．0ml／min。结果：MPA、MPAG血药浓度线性范围分别为1．0～50μg／ml（r=0．9994，n=6）和2．5～100μg／ml（r=0．9995，n=7），MPA、MPAG血浆最低检测浓度分别为 0．5 μg/ml和 1．0 μg／ml，高、中、低 3种浓度 MPA平均回收率分别为（95．75±2．31）％、（104．10±1．91）％和（98．11±4．24）％，MPAG则分别为（97．37±1．43）％、（101．10±5．41）％和（105．44±7．59）％。结论：本研究为MPA和MPAG的药代动力学研究及血药浓度监测提供了一种测定方法。
Objective: To establish a method for the determination of mycophenolic acid (MPA) and glucuronide (MPAG) in plasma. Methods: The plasma concentration was determined by high performance liquid chromatography (HPLC) - diode array detector with detection wavelength of 254 nm. Blood samples plus acetonitrile precipitated protein. Column: Hypersil ODS2 (200 min × 4.6 mm, 5 μm); Mobile phase: 25% CH3CN + 75% 0.02 mol / L KH2PO4. (PH 3.0), phase B 70% CH3CN + 30% 0.02 mol / L K2HPO4 (pH 6.5), gradient elution; flow rate: 1.0 ml / min. Results: The linear ranges of MPA and MPAG were 1.0-50μg / ml (r = 0.9994, n = 6) and 2.5-100μg / ml (r = 0.9995, n = 7) The MPA and MPAG plasma concentrations were 0.5 μg / ml and 1.0 μg / ml respectively. The average recoveries of MPA and MPAG were (95.75 ± 2.31)%, (104.75 ± 2.31)%, (97.11 ± 1.43)%, (101.10 ± 5.41)% and (105.44)%, (98.11 ± 4.24)% and ± 7.59)%. Conclusion: This study provides a method for the pharmacokinetic study and plasma concentration monitoring of MPA and MPAG.