论文部分内容阅读
目的:探究扇贝多肽(PCF)抗紫外线UVA对无毛小鼠皮肤氧化损伤的作用.方法:昆明种无毛小鼠,随机分为双蒸水未照射组和模型组(双蒸水照射组、5％PCF组、20％PCF组、10％维生素 C组).酶法测定皮肤匀浆抗氧化酶(GSH-Px、T-AOC、SOD)活性和MDA的含量;免疫组织化学法测定皮肤Bc1-2和NOS蛋白表达;电镜观察皮肤组织超微结构.结果:PCF能明显增加皮肤组织匀浆总抗氧化能力及GSH-Px、SOD活性,降低MDA含量.免疫组化结果表明,PCF能上调Bcl-2蛋白的表达;抑制NOS蛋白的表达.超微结构显示20％PCF组表皮细胞结构正常,成纤维细胞的细胞器结构正常;模型对照组表皮细胞损伤,胞质内可见空泡形成,真皮成纤维细胞内可见囊泡状扩张的滑面内质网,粗面内质网等细胞器减少,PCF组与模型对照组比较各项指标均有改善(P<0.05).结论:扇贝多肽具有抗紫外线UVA对无毛小鼠皮肤氧化损伤的作用.其机制与扇贝多肽上调BCl-2蛋白表达,下调NOS蛋白的表达,提高抗氧化酶含量,抑制脂质过氧化有关.
Objective: To investigate the effect of scallop polyp (PCF) UVA on skin oxidative damage in hairless mice.Methods Kunming hairless mice were randomly divided into untreated and untreated groups and model group (double distilled water, 5% PCF group, 20% PCF group and 10% vitamin C group). The activity of GSH-Px, T-AOC and SOD and the content of MDA in the skin homogenate were measured by enzymatic method. 2 and NOS protein were detected by electron microscopy.Results: PCF could significantly increase the total antioxidant capacity and the activities of GSH-Px and SOD, and decrease the content of MDA in skin homogenate.Immunohistochemistry results showed that PCF increased The expression of Bcl-2 protein and NOS protein were detected by immunohistochemistry.The ultrastructure showed that the epithelial cell structure was normal in 20% PCF group and the organelle structure was normal in fibroblasts.The epidermis cells in the model control group were damaged, vacuoles were formed in the cytoplasm, Fibroblasts can be seen vesicle-like expansion of sliding endoplasmic reticulum, rough endoplasmic reticulum and other organelles decreased PCF group compared with the model control group were improved indicators (P <0.05) .Conclusion: Scallop polypeptide has anti Effect of UVA on skin oxidative damage in hairless mice Scallop peptides up-regulate BCl-2 protein expression, down-regulate the expression of NOS protein, increase antioxidant enzyme content and inhibit lipid peroxidation.