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甲单向一步SDS-PAGE方法分析表明亲本品种Suneca和Cook在麦谷蛋白亚基的5个位点(Glu-B1,Glu-D1,Glu-A3,Glu-B3和Glu-D3)均含不同等位基因。本研究重点对Suneca×Cook的F_4代群体中在麦谷蛋白亚基位点均为纯合基因的60个系的出粉率(FY),面粉蛋白质含量(FP)及和面时间(PTM)进行了分析,以研究麦谷蛋白各亚基位点等位基因变异及位点间互作对小麦品质特性的影响。结果表明,不同基因型间出粉率无显著差异,Glu-D1位点等位基因d和a对FP的效应存在显著差异,Glu-Dld基因(编码5+10亚基)的正效应显著高于Glu-Dla基因(编码2+12亚基);Glu-D1、Glu-A3和Glu-B3位点上基因的等位变异对PTM有显著和极显著影响,含Glu-Dld、Glu-A3b和Glu-B3b基因的系分别比含Glu-Dla,Glu-A3d和Glu-B3h基因的系有较长的和面时间;Glu-B1位点上等位变异i和u以及Glu-D3位点等位基因b和e分别对PTM无明显影响。在这种遗传背景下,麦谷蛋白亚基位点对PTM的效应大小依次排列为Glu-D1>Glu-B3>Glu-A3>GIu-B1=Glu-D3。Glu-1位点和Glu-3位点间对和面特性的影响存在累加效应和互作效应。
A one-step SDS-PAGE analysis showed that the parents Suneca and Cook had different identities (Glu-B1, Glu-D1, Glu-A3, Glu-B3 and Glu- D3) at all five glutenin subunits Bit gene. The focus of this study was on FY, flour protein content (FP) and surface area time (PTM) for the 60 lines of homozygous genes in the F_4 population of Suneca × Cook In order to study the allelic variation of each subunit of glutenin and the interaction between the loci on the quality characteristics of wheat. The results showed that there was no significant difference in the yield of wheat between different genotypes. The effects of allele d and a on FP were significantly different at Glu-D1 locus, and the positive effect of Glu-Dld gene (encoding 5 + 10 subunit) Allele variation at Glu-D1, Glu-A3 and Glu-B3 loci had significant and extremely significant effects on PTM, including Glu-Dld, Glu-A3b And Glu-B3b genes had longer and longer surface time than the lines containing Glu-Dla, Glu-A3d and Glu-B3h, respectively; alleles i and u and Glu-D3 at Glu-B1 Alleles b and e had no significant effect on PTM. In this genetic background, the effects of glutenin subunit loci on PTM were ranked as Glu-D1> Glu-B3> Glu-A3> GIu-B1 = Glu-D3. There are additive and interaction effects on the surface properties of Glu-1 and Glu-3.