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用籼稻IR64与粳稻Azucena杂交发展的水稻双单倍体(DH)群体(共123个系)作试验材进行溶液培养试验。试验设两个处理:K正常供应(40mp/kg)和低K胁迫(5mg/kg)。培养4周后,测定参数包括株高,分蘖数,茎干重,根干重与植株K含量;K吸收量与K利用率根据测定参数计算。175个标记在12条染色体上的分子标记用于耐低钾胁迫数量性状位点分析。耐低钾胁迫数量性状位点定义为仅在低K胁迫条件下检测到的QTLs(低K胁迫诱导QTLs)。分析结果表明,sd-1位点在K充分供应与低K胁迫条件下均对株高有重要影响;但在低K胁迫下检测到4个影响株高的QTLs和3个影响分蘖数的QTLs。K正常供应条件下,影响株高的sd-1位点对其他参数均有重要影响,但在K胁迫条件下没有发现这种影响。检测到的3个影响茎干重和根干重的低K胁迫诱导QTL相同,其中一个QTLs也同时影响植株K含量、K吸收量和K利用率,另有两个QTLs对植株K含量也有影响。与检测到的低K胁迫诱导QTLs联锁的标记基因型参数平均数比较表明,除K利用率外,IR64基因型均优于Azucena基因型。本研究结果对进一步深入研究水稻耐低K胁迫遗传背景提供了参考。
A double-haploid (DH) population (123 lines in total) developed from a cross between indica rice IR64 and japonica rice Azucena was used as a test material for solution culture. Two treatments were set for the trial: K normal supply (40 mp / kg) and low K stress (5 mg / kg). After 4 weeks of culture, the parameters of plant height, tiller number, stem weight, root dry weight and plant K content were determined. The K uptake and K utilization rate were calculated according to the measured parameters. The molecular markers of 175 markers on 12 chromosomes were used for quantitative trait loci analysis of low potassium tolerance. Quantitative trait loci resistant to low potassium stress were defined as QTLs (low Q stress induced QTLs) detected only under low K stress. The results showed that sd-1 locus had an important effect on plant height under the condition of adequate K supply and low K stress. Four QTLs affecting plant height and three QTLs affecting tillering number were detected under low K stress . Under the normal supply condition of K, the sd-1 locus affecting plant height had an important influence on other parameters, but no such effect was found under K stress. Three QTLs were detected under the low K stress, which affected the stem and root dry weight. One of the QTLs also affected the K content, K uptake and K utilization of plant, and the other two QTLs also affected plant K content . The average number of marker genotype parameters interlocked with the detected low-K stress-induced QTLs showed that the IR64 genotype was superior to the Azucena genotype except K utilization. The results of this study provide a reference for further studying the genetic background of low K tolerance in rice.