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测定味精中谷氨酸含量,多采用旋光法和中和法。由于氨基酸在碱性条件下能定量地从磷酸铜沉淀中络合铜,所以可通过原子吸收光谱法测定铜间接测定味精中谷氨酸含量。所拟方法简便,重现性好,可检出0.10mg谷氨酸,变异系数为2.62～4.71%,回收率在92～109%。用中和法与本法同时测定一些味精样品中谷氨酸含量,经统计处理,无显著差异。一仪器与试剂 GFU-202型原子吸收光谱仪;铜空心阴极灯饱和氯化钾溶液 NaOH-硼酸-KCl缓冲溶液:pH 9.0,取0.1MNaOH溶液21.4ml和0.2M硼酸与0.2M KCl混合液25ml混合均匀(0.2M硼酸与0.2M KCl混合液:
Determination of glutamate in MSG content, and more use of optical rotation and neutralization method. Since amino acids can be quantitatively complexed with copper from a copper phosphate precipitate under alkaline conditions, the content of glutamic acid in the indirect determination of copper can be determined by atomic absorption spectrometry. The proposed method is simple, reproducible, 0.10mg glutamic acid can be detected, the coefficient of variation of 2.62 ~ 4.71%, the recovery rate of 92 ~ 109%. The determination of glutamic acid in some MSG samples by neutralization method and this method was not statistically significant. An instrument and reagent GFU-202 atomic absorption spectrometer; copper hollow cathode lamp saturated potassium chloride solution NaOH-boric acid-KCl buffer solution: pH 9.0, take 0.1M NaOH solution 21.4ml and 0.2M boric acid and 0.2M KCl mixture 25ml mixed Uniform (0.2 M boric acid and 0.2 M KCl mixture: