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目的:研究血管紧张素转化酶抑制剂(ACEI)对兔腹主动脉球囊损伤后的影响及作用机制。方法:雄性新西兰大白兔随机分为ACEI组和对照组,每组10只。球囊损伤腹主动脉后,ACEI组给予福辛普利5mg.kg-1.d-1,对照组不给予药物处理。实验4周后,取腹主动脉标本,使用计算机辅助形态分析系统比较2组新生内膜、中膜及管腔面积,测定血清、组织肝细胞生长因子(HGF)浓度以及与新生内膜面积的相关关系。结果:ACEI组新生内膜面积显著低于对照组[(0.048±0.011)mm2∶(0.124±0.021)mm2,P<0.001];管腔面积对照组显著低于ACEI组[(1.920±0.140)mm2∶(2.290±0.260)mm2,P=0.001];血管中膜面积2组差异无统计学意义[(0.890±0.130)mm2∶(0.990±0.120)mm2,P>0.05]。2组血浆HGF浓度差异无统计学意义[(485±84)ng/L∶(507±106)ng/L,P>0.05],组织HGF浓度ACEI组显著高于对照组[(49.9±5.5)ng/g∶(32.7±4.5)ng/g,P<0.001];单因素相关分析发现新生内膜面积与组织HGF浓度呈负相关(r=-0.943,P<0.001)。结论:ACEI可以减轻兔腹主动脉球囊损伤后新生内膜的形成,增加管腔面积,升高组织HGF浓度而对血浆HGF浓度无影响。ACEI减轻血管球囊损伤后的新生内膜形成部分通过升高组织HGF发挥作用。
Objective: To investigate the effect and mechanism of angiotensin converting enzyme inhibitor (ACEI) on the injury of abdominal aorta balloon in rabbits. Methods: Male New Zealand white rabbits were randomly divided into ACEI group and control group, with 10 in each group. Balloon injury abdominal aorta, ACEI group given fosinopril 5mg.kg-1.d-1, the control group did not give drug treatment. Four weeks after the experiment, the specimens of abdominal aorta were taken and the neointima, media and luminal area were compared by computer-aided morphological analysis system. The serum and tissue concentrations of hepatocyte growth factor (HGF) and the ratio of neointimal area relationship. Results: The area of neointima in ACEI group was significantly lower than that in control group [(0.048 ± 0.011) mm2:(0.124 ± 0.021) mm2, P <0.001]; the area of the luminal area in control group was significantly lower than that in ACEI group [(1.920 ± 0.140) mm2 : (2.290 ± 0.260) mm2, P = 0.001]. There was no significant difference in the area of vascular intima between the two groups [(0.890 ± 0.130) mm2: (0.990 ± 0.120) mm2, P> 0.05]. There was no significant difference between the two groups in plasma HGF concentration [(485 ± 84) ng / L: (507 ± 106) ng / L, P> 0.05] ng / g: (32.7 ± 4.5) ng / g, P <0.001]. The single factor correlation analysis showed that the neointimal area was negatively correlated with the tissue HGF concentration (r = -0.943, P <0.001). CONCLUSION: ACEI can attenuate the formation of neointima in rabbit abdominal aorta after balloon injury, increase the luminal area and increase the concentration of HGF in the tissue, but have no effect on the concentration of plasma HGF. ACEI attenuates neointimal formation following vascular balloon injury by increasing tissue HGF.