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目的：探讨抑癌基因p16的表达与前列腺癌发生、发展的关系，从而从翻译水平研究p16基因与前列腺癌生物学行为的关系．方法：采用免疫组织细胞化学SABC染色法技术、兔抗人pl6多克隆抗体（C-20）和组织抗原修复技术（SanyoEM-715FW微波炉的“慢速烹调、高火力档”15’）对41例前列腺癌标本进行检测p16基因蛋白的表达结果：①在41例前列腺癌组织标本中，蛋白的阳性表达率（为48．8％）较高．提示p16基因的突变或缺失可能在前列腺癌的发生发展中具有重要作用．SABC法免疫组化的结果表明，P16蛋白均表达在前列腺癌腺上皮细胞的细胞质中，大部分呈弥漫均匀分布，部分呈区域性表达．②p16基因蛋白低表达是病理分级高（恶性程度高）、具有侵袭性前列腺癌的重要特征，是其晚期事件．pl6基因蛋白表达率在病理Ⅰ，Ⅱ，Ⅲ级分别为80％，474％，25％，差异显著（P＜0．05）;在局限于前列腺包膜内的A＋B期为65．4％，侵袭包膜外及扩散、转移的C＋D期为20％，差异显著（P＜O．05）;在死亡组为卫2．5％，存活组为61．5％，差异显著（P＜O．05），预示检测P16蛋白可作为判断前列腺癌预后的一个指标．结论：p16基因与前列腺癌的发生、发展密切相关;参与前列腺癌的自然病程;p16蛋白的检测是判定前列腺癌预后的一个有用的指
OBJECTIVE: To investigate the relationship between the expression of tumor suppressor gene p16 and the occurrence and development of prostate cancer so as to study the relationship between the p16 gene and the biological behavior of prostate cancer at the translation level. Methods: Immunohistochemical SABC staining method, rabbit anti-human pl6 polyclonal antibody (C-20) and tissue antigen retrieval technology (SanyoEM-715FW microwave oven "slow cooking, Prostate cancer specimens were detected p16 gene protein expression results: ① in 41 cases of prostate cancer specimens, the positive rate of protein (48.8%) higher. Suggesting that the mutation or deletion of p16 gene may play an important role in the development of prostate cancer. The results of SABC immunohistochemistry showed that P16 protein was expressed in the cytoplasm of prostate glandular epithelial cells, most of which were diffusely distributed and partially expressed. ② The low expression of p16 gene protein is a high pathological grade (high degree of malignancy), with an important feature of invasive prostate cancer is the late event. The expression of pl6 protein in pathological grade Ⅰ, Ⅱ and Ⅲ were 80%, 474% and 25%, respectively (P <0.05). The A + B phase in the prostatic capsule was 65.4% There was a significant difference (P <0.05) in the C + D stage of invasive and diffusing, metastatic C + D stage, with a difference of 2.5% in the death group and 61.5% in the survival group (P <0.05). 05), indicating that the detection of P16 protein can be used as an indicator to judge the prognosis of prostate cancer. Conclusion: The p16 gene is closely related to the occurrence and development of prostate cancer. It is involved in the natural course of prostate cancer. The detection of p16 protein is a useful indicator of the prognosis of prostate cancer