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研究氮能神经和平滑肌肌动蛋白（Ａｃｔｉｎ）在膀胱壁和膀胱三角区的分布及其对膀胱功能的影响。采用免疫组化法测定开放手术良性前列腺增生（ＢＰＨ）病人逼尿肌和膀胱三角区平滑肌及无膀胱出口、尿道梗阻病人逼尿肌中Ａｃｔｉｎ、一氧化氮合酶（ＮＯＳ）和神经原特异性醇化酶（ＮＳＥ）、ＮＯＳ神经分布。结果显示：正常和梗阻后逼尿肌及梗阻后三角区平滑肌中有大量的ＮＳＥ和ＮＯＳ阳性神经节。ＮＯＳ阳性神经数在正常与梗阻后逼尿肌、正常与低顺应性逼尿肌和正常顺应性与低顺应性逼尿肌之间均有显著性差异（Ｐ＝０．０１１，０．００６，０．０３４）。ＮＯＳ阳性神经与ＮＳＥ阳性神经比率正常逼尿肌显著高于梗阻后的逼尿肌（Ｐ＝００４０）。正常与梗阻逼尿肌的Ａｃｔｉｎ表达之间有显著性差异（Ｐ＝０．００２）。结论：梗阻后的去神经现象中氮能神经的去神经是优势现象；氮能神经去神经后可以影响膀胱的贮尿和排尿功能，引起膀胱顺应性的改变和排尿的异常。
To investigate the distribution of Actin in bladder wall and bladder trigone and its effect on bladder function. Immunohistochemical method was used to detect Actin, nitric oxide synthase (NOS) and neuron specificity in detrusor of patients with open surgery benign prostatic hyperplasia (BPH), detrusor and bladder trigone smooth muscle and without bladder outlet, urethral obstruction Alcoholase (NSE), NOS nerve distribution. The results showed that there were a large number of NSE and NOS positive ganglia in normal and obstructed detrusor and obstructed triangle smooth muscle. NOS positive nerve detrusor after normal and obstruction, normal and low compliance detrusor and normal compliance and low compliance detrusor were significantly different (P = 0.011,0.006, 0.034). The ratio of NOS-positive neurons to NSE-positive neurons in normal detrusor was significantly higher than that in detrusor after obstruction (P = 0.040). There was a significant difference in Actin expression between normal and obstructive detrusor (P = 0.002). CONCLUSION: The denervation of Nerve nerve in obstructive denervation is the predominant phenomenon. Nerve denervation can affect urinary bladder urination and urinary bladder function, causing bladder compliance changes and voiding abnormalities.