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目的 :研究 VLA- 5及 VLA- 6是否参与内皮细胞促进的造血干 /祖细胞定向移行。方法 :对纯化人 CD34+ 细胞进行体外移行及阻断实验 ,观察其穿移覆盖人脐静脉内皮细胞 ( HUVECs)滤膜的能力。应用四色荧光活化流式细胞术 ( FACS)检测 CD34+细胞其粘附分子及趋化因子受体CXCR- 4的表达谱。结果 :基质由来因子 ( SDF) - 1 α介导的动员外周血 ( m PB)及骨髓 ( BM)来源的CD34+ 细胞穿透覆盖 HUVECs滤膜百分率分别为 ( 5 6.6± 2 0 .1 ) %及 ( 1 5 .6± 1 .8) % ,显著高于其穿移未覆盖 HUVECs滤膜的比率。预先对 CD34+ 细胞进行抗 VLA- 5和 /或 VLA- 6中和抗体处理可消除这一促进效应。此外 ,BM来源的 CD34+细胞其穿移覆盖及未覆盖 HUVECs滤膜的能力均显著低于 m PB CD34+细胞 ,两者间穿移能力的差异与其 VLA- 5及 VLA- 6(而非 VLA- 4及趋化因子受体 CXCR- 4)抗原表达水平相关。结论 :VLA- 5和 VLA- 6参与 HUVECs促进 HS/PCs穿移能力。
OBJECTIVE: To investigate whether VLA-5 and VLA-6 are involved in endothelial cell-directed hematopoietic stem / progenitor cell migration. Methods: Purified human CD34 + cells were subjected to in vitro migration and blocking experiments. The ability of the purified human CD34 + cells to penetrate the membrane of human umbilical vein endothelial cells (HUVECs) was observed. Four-color fluorescence-activated flow cytometry (FACS) was used to detect the expression of chemokine CXCR-4 on CD34 + cells. Results: The percentages of penetrated HUVECs in mobilized peripheral blood (m PB) and bone marrow (BM) derived from stromal factor (SDF) -1 α were (5 6.6 ± 2.0 0.1)% and (15.6 ± 1.8%), which was significantly higher than that of the uncoated HUVECs. Pretreatment of CD34 + cells with anti-VLA-5 and / or VLA-6 neutralizing antibodies abolished this promoting effect. In addition, BM-derived CD34 + cells were significantly less capable of penetrating and uncovering HUVECs than m PB CD34 + cells, with a difference in their ability to migrate between VLA-5 and VLA-6 (not VLA-4 And chemokine receptor CXCR-4) antigen expression level. Conclusion: VLA-5 and VLA-6 are involved in HUVECs promoting HS / PCs migration.