Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with

• 作 者: 李奇芬 朱纯吾 张士敏 王慧芬
• 机 构: Department of Infectious Diseases Southwest Hospital,Third Military College,Chongqing,Department of
• 出 处: Journal of Medical Colleges of PLA
• 发表日期: 1988年02期
• 关键词: DNA Probe Hybridization occasionally grains minutes paraffin sections cytoplasm

The in situ hybridization of HBV DNA with biotinylated probe informalin-fixed and paraffin-embedded liver tissue sections was performed and thelocalization and morphology of HBV DNA in various types of chronic liver diseases wereobserved.25 out of the 75 cases studied were HBV DNA positive (33.3%).HBV DNA inhepatocytes was demonstrated as numerous coarse grains in cytoplasm and occasionally afew grains in individual nuclei.The distribution of the HBV DNA positive hepatocytes inthe sections was in 3 types,i.e.,the diffuse type (Type I,4/25,16%),the focal type (Type Ⅱ,20/25,80%),and the scattered type (Type Ⅲ,1/25,4%).The HBV DNA grains incytoplasm might be condensed (Type a) or sparsed (Type b) depending on the number of theHBV DNA grains.The different distribution patterns and the amount of HBV DNAgrains might be a reflection of the replication activity and the state of infectivity of HBV.HBV DNA positive hepatocytes were found only in the tissues contiguous to the cancertissue in cases with hepatocellular carcinoma with cirrhosis.

The in situ hybridization of HBV DNA with biotinylated probe informalin-fixed and paraffin-embedded liver tissue sections was performed and the localization and morphology of HBV DNA in various types of chronic liver diseases wereobserved.25 out of the 75 cases studied were HBV DNA positive ( 33.3%). HBV DNA in hepatocytes were demonstrated as massive large grains in cytoplasm and occasionally a few grains in individual nuclei. The distribution of the HBV DNA positive hepatocytes inthe sections was in 3 types, ie, the diffuse type (Type I, 4/25 , The focal type (Type II, 20/25, 80%), and the scattered type (Type III, 1 / 25,4%). The HBV DNA grains incytoplasm might be condensed (Type a) or sparsed (Type b) depending on the number of the HBV DNA grains. The different distribution patterns and the amount of HBV DNAgrains might be a reflection of the replication activity and the state of infectivity of HBV. HBV DNA positive hepatocytes were found only in the tissues contiguous to the cancertissue in cases with hepatocellular carcinoma with cirrhosis.