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目的：探讨白细胞介素２（ＩＬ－２）和白细胞介素６（ＩＬ－６）基因疗法的抗肿瘤作用机制。方法：将脂质体包裹的ＩＬ－２基因和ＩＬ－６基因直接注射至ＥＬ－４淋巴瘤小鼠腹腔后，研究淋巴瘤小鼠的腹腔巨噬细胞（Ｍφ）在基因治疗后的功能变化。结果：腹腔内单独注射脂质体包裹的ＩＬ－２基因后，可明显提高Ｍφ的ＭＨＣＩａ的表达，增强Ｍφ的杀伤活性，并促进Ｍφ诱导白细胞介素１（ＩＬ－１）及肿瘤坏死因子（ＴＮＦ）的产生；腹腔内单独注射脂质体包裹的ＩＬ－６基因后，对Ｍφ也具有激活作用，但效果不及前者。二者联合后能非常显著地提高荷瘤小鼠的Ｍφ的上述功能。结论：腹腔内脂质体介导的ＩＬ－２基因和ＩＬ－６基因治疗能有效地解除淋巴瘤小鼠体内Ｍφ功能的抑制，激活Ｍφ发挥抗肿瘤作用。
Objective: To investigate the antitumor mechanism of interleukin 2 (IL-2) and interleukin 6 (IL-6) gene therapy. Methods: The IL-2 gene and IL-6 gene encapsulated in liposomes were directly injected into the peritoneal cavity of EL-4 lymphoma mice to study the functional changes of peritoneal macrophages (Mφ) in lymphoma mice after gene therapy . Results: After intraperitoneal injection of liposome-encapsulated IL-2 gene alone, the expression of MHCIa of Mφ was significantly increased, the cytotoxicity of Mφ was enhanced and the expression of interleukin 1 (IL-1) and tumor necrosis factor-α TNF). After intraperitoneal injection of liposome-encapsulated IL-6 gene alone, Mφ also had an activation effect, but the effect was not as good as the former. The combination of the two can very significantly improve Mφ in tumor-bearing mice. Conclusion: Intraperitoneal liposome-mediated IL-2 gene and IL-6 gene therapy can effectively relieve the inhibition of Mφ function in lymphoma mice and activate Mφ to exert anti-tumor effect.