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酯酶是一组重要的药物代谢酶,它不仅使许多药物转变成无活性的代谢产物,而且也可使多种药物前身转变成有活性的形式。本文对16例病人肝活检组织进行检测,探讨年龄与肝脏阿斯匹林水解的关系。肝活检组织均来源于无心、肝、肾疾病和未服用有影响酶活性药物的病人(男、女各8例,年龄45～88岁)。肝标本通过用冰冷缓冲液均质、离心等程序制成50μl含有后线粒体、细胞质、微粒体三种成份的磷酸盐缓冲液并与30μl新鲜制备的乙酰水杨酸液混合,用HPLC测定阿斯匹林酯酶活性。用Lowry
Esterase is an important set of drug-metabolizing enzymes that not only convert many drugs into inactive metabolites, but also transform many drug precursors into active forms. In this paper, 16 cases of patients with liver biopsy to detect the relationship between age and hepatic aspirin hydrolysis. Liver biopsy were derived from heartless, liver and kidney disease and did not take drugs that affect enzyme activity (8 males and 8 females, aged 45 to 88 years old). Liver samples were prepared by mixing 50 μl of a phosphate buffer containing the following three components of mitochondria, cytoplasm and microsomes by homogenization with an ice-cold buffer, centrifugation, and the like, and mixing with 30 μl of freshly prepared acetylsalicylic acid solution, Aspirin activity. With Lowry