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目的:探讨14-3-3σ蛋白在子宫肌瘤中的表达及其临床意义。方法:采用RT-PCR及Western blot法分别对20例子宫肌瘤患者的肌瘤组织和同源正常肌层组织中的14-3-3σ表达情况进行半定量检测。结果:在20例子宫肌瘤患者的子宫肌瘤组织和同源正常肌层组织中,RT-PCR及Western blot法均检测到14-3-3σ基因表达,子宫肌瘤组织中出现明显的低表达或表达缺失。结论:14-3-3σ蛋白的低表达与子宫肌瘤的发生相关。
Objective: To investigate the expression of 14-3-3σ protein in uterine fibroids and its clinical significance. Methods: The expression of 14-3-3σ in myometrium and myometrial tissues of 20 patients with uterine leiomyoma was detected by RT-PCR and Western blot respectively. Results: The expression of 14-3-3σ gene was detected by RT-PCR and Western blot in 20 cases of myoma of uterine fibroids and in the normal myometrium. The expression of 14-3-3σ gene was significantly lower in uterine fibroids Lack of expression or expression. Conclusion: The low expression of 14-3-3σ protein is associated with the occurrence of uterine fibroids.