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Objective: To study the effect of acupuncture on serum glucagon (GL) and immuno-cytokines in diabetes type-Ⅱ patients. Methods: A total of 120 diabetes type-Ⅱ patients including 70 inpatients and 50 outpatients were randomly divided into simple acupuncture group (n=18), acupuncture plus medication group (n = 52) and simple medi cation group (n=50). Acupoints used were Pishu (BL 20). Yishu, Shenshu (BL 23), Qihai (CV 6), Quchi (LI 11), Neiguan (PC 6). Zusanli (ST 36). Sanyinjiao (SP 6). etc. and medicines used were Glipizide (2.5-5 mg, t. i. d.), Dimethyldiguanide hydrochloride (0.25-0.5 g, t. i. d.), etc.. Serum GL, tumor necrosis factor (TNF)-α and inter lukin(IL)-6 contents were determined with radioimmunoassay, and blood sugar was also detected with routine method. Results: After treatment, serum GL, TNF-α and IL-6 contents declined significantly in comparison with pre-treatment (P <0. 05, 0. 01), and the effect of acupuncture plus medication group is superior to those of simple acpuncture or medication groups, particularly in lowering serum GL (P <0.01). Conclusion: The regulative action of acupuncture on blood GL and immuno-cytokine levels maybe contribute to its functions in stabilizing blood sugar and preventing complications.