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目的:观察附子对大鼠坐骨神经分支选择性损伤(SNI)模型神经病理性疼痛的镇痛作用。方法:成年雄性SD大鼠60只随机分为5组(n=12),建立SNI模型,于术后第5天分别给予生理盐水1mL(组Ⅰ),附子0.5g/kg(组Ⅱ),1g/kg(组Ⅲ),2g/kg(组Ⅳ),4g/kg(组Ⅴ)溶于1mL生理盐水中灌胃,观察给药前和给药后0.5、1、2、3h不同时间点各组大鼠机械性缩足反射阈值(MWT)及热缩足反射维持时间(TWD)的测定值,各组大鼠分别于术后7、14、21天处死大鼠,并取大鼠腰段脊髓采用双抗体夹心ELISA法测定细胞因子的含量。结果:与组Ⅰ比较,给药1h后组Ⅲ,Ⅳ,Ⅴ的MWT升高,且TWD缩短;组Ⅱ的MWT及TWD差异无统计学意义。组Ⅲ,Ⅳ,Ⅴ于术后7、14、21天的细胞因子TNF-α,IL-1β和IL-6的含量明显低于组Ⅰ,组Ⅱ术后21天的细胞因子含量低于组Ⅰ。结论:附子能明显缓解SNI大鼠的机械和热痛觉过敏现象,达到镇痛效果,同时能使脊髓细胞因子的表达减少。
Objective: To observe the analgesic effect of aconite on neuropathic pain in rats with sciatic nerve branch selective injury (SNI) model. Methods: Sixty adult male Sprague-Dawley rats were randomly divided into 5 groups (n = 12). SNI models were established. On the 5th postoperative day, 1 mL saline (group Ⅰ) and 0.5 g / kg aconite (group Ⅱ) 1 g / kg (group Ⅲ), 2 g / kg (group Ⅳ), 4 g / kg (group Ⅴ) were dissolved in 1 mL of normal saline, and were observed before administration and 0.5,1,2,3 h after administration The rats were sacrificed at 7, 14 and 21 days after operation respectively, and the rats were sacrificed Segment spinal cord using double antibody sandwich ELISA assay cytokine content. Results: Compared with group Ⅰ, the MWTs of group Ⅲ, Ⅳ and Ⅴ increased and the TWD shortened after 1 hour of administration. The differences of MWT and TWD in group Ⅱ were not statistically significant. The levels of cytokines TNF-α, IL-1β and IL-6 in group Ⅲ, Ⅳ and Ⅴ at 7, 14 and 21 days after operation were significantly lower than those in group Ⅰ. The levels of cytokines at 21 days after operation in group Ⅱ were lower than those in group Ⅱ Ⅰ. Conclusion: Aconite can obviously relieve the mechanical and thermal hyperalgesia in SNI rats, and achieve the analgesic effect, meanwhile reduce the expression of cytokines in the spinal cord.