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目的观察猪带绦虫虫卵实验感染中间宿主家猪后囊尾蚴致肝纤维化作用,并对病变程度进行定量分析。方法实验猪分成3组,将亚洲猪带绦虫虫卵以高剂量(60 000个/ml)、中剂量(40 000个/ml)和低剂量(10 000个/ml)分别灌服感染家猪,每种剂量感染2头。健康家猪2头为对照组。30 d后剖杀,取肝脏,制作病理切片,HE和Cason染色,对实验猪肝脏病变组织进行病理变化观察及胶原纤维含量分析。结果与健康对照组比较,实验组家猪肝组织发生不同程度的变性、坏死、炎细胞侵润,肝、脾指数增加(P<0.05或P<0.01);肝纤维化改变明显,胶原纤维总面积和总吸光度值增加,胶原纤维含量显著增加(P<0.05或P<0.01)。结论猪带绦虫虫卵感染家猪后以肝脏囊尾蚴寄生居多,同时发生以肉芽肿和纤维化改变为主的病理损害且较为严重。
Objective To observe the effect of experimental infection of Taenia solium on hepatic fibrosis induced by Cysticercus cellulosae in middle host pigs, and quantitatively analyze the degree of lesion. Methods The experimental pigs were divided into 3 groups. The infected pigs were challenged with high dose (60 000 / ml), medium dose (40 000 / ml) and low dose (10 000 / ml) , 2 for each dose. Two healthy pigs served as the control group. Thirty days later, the rats were sacrificed, the liver was taken out, pathological sections were made, HE and Cason were stained to observe the pathological changes of liver tissues and collagen fibers in experimental pigs. Results Compared with the healthy control group, the liver tissue of experimental pigs had different degree of degeneration, necrosis, infiltration of inflammatory cells, increased liver and spleen index (P <0.05 or P <0.01), obvious changes of hepatic fibrosis, Area and total absorbance increased, collagen fibers increased significantly (P <0.05 or P <0.01). Conclusion The results showed that the majority of C. cysticercus parasites were infected with Taenia solium eggs in domestic pigs, and pathological lesions were mainly granulomatous and fibrosis.