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应用ｒａｓ癌基因产物Ｐ２１蛋白一段氨基酸序列的合成肽的单克隆抗体ＥＢ２４１１和ｅｒｂＢ２癌基因产物Ｐ１８５抗体，对人胃癌、癌旁和正常组织中ｒａｓ和ｅｒｂＢ２的表达进行免疫组化研究，结果：Ｐ２１在正常、癌旁和癌组织的阳性率分别为１３３％、７９３％和６８９％；阳性细胞数分别为（４０±１５），（２１９±１０４）和（１４７２±４００）ｍｍ－２，三者之间呈显著性差异（Ｐ＜００１）。Ｐ２１的表达与肿瘤的分化类型无显著性差异。早期胃癌（病变局限于粘膜层和粘膜下层）Ｐ２１表达高于进展期胃癌。高分化腺癌中Ｐ１８５ＩＲ阳性细胞数明显多于中、低分化癌。Ⅰ、Ⅱ期胃癌Ｐ１８５阳性细胞数较少，而Ⅲ、Ⅳ期显著增多。提示：Ｐ２１参与胃癌的早期发生，而Ｐ１８５与胃癌的晚期发展有关，并参与胃癌细胞的分化调节。
Application ras oncogene P21 protein amino acid sequence of a synthetic peptide monoclonal antibody EB2411 and erbB 2 oncogene product P185 antibody to human gastric cancer, adjacent and normal tissue ras and erbB 2 immunohistochemical study Results: The positive rate of P21 was 133%, 793% and 689% respectively in normal tissues, adjacent cancerous tissues and cancerous tissues. The positive cell numbers were (40 ± 15) 9 ± 104) and (1472 ± 400) mm-2, respectively (P <001). P21 expression and tumor differentiation type no significant difference. The expression of P21 in early stage gastric cancer (the lesion is confined to the mucosa and submucosa) is higher than that of advanced gastric cancer. P185 IR positive cells in well-differentiated adenocarcinoma was significantly more than in moderate and poorly differentiated carcinoma. The number of P185 positive cells in stage Ⅰ and Ⅱ gastric cancers was less, while the stage Ⅲ and Ⅳ were significantly increased. Tip: P21 is involved in the early occurrence of gastric cancer, while P185 is associated with the advanced development of gastric cancer and is involved in the regulation of differentiation of gastric cancer cells.