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目的:本研究想寻找泔水油的特有靶标,为下一步研制快速检测泔水油试剂奠定基础。方法:本实验对泔水样品采用传统方法进行细菌培养、分纯和生化鉴定细菌种类,然后计数每份样品各典型菌落数量,确定出优势菌种;再用鲎试剂凝胶法对比正常食用油和泔水油里面的脂多糖含量。结果:30份样品,检出大肠埃希菌26株,肺炎克雷伯菌肺炎亚种15株,在单个样品中这两种菌的菌落数量也比其它菌多。鲎试验对比中发现:泔水油中细菌代谢产物--脂多糖含量比正常食用油的高出100-1000倍。结论:确定泔水中的优势菌种为肺炎克雷伯肺炎亚种和大肠埃希菌。细菌代谢产物——脂多糖可以作为一项泔水油的抗原检测靶标。
Objectives: This study is to find the unique target of Hydra oil, which will lay the foundation for the next step of developing Hydrolyzed Hydrocarbon reagent. Methods: In this experiment, the traditional method was used to culture the water samples, and the bacterial species were identified by pure and biochemical methods. Then, the number of typical colonies per sample was counted to determine the dominant species. Then the gel method was used to compare with the normal edible oil and泔 water oil inside the lipopolysaccharide content. Results: Among 30 samples, 26 strains of Escherichia coli and 15 strains of Klebsiella pneumoniae pneumonia were detected. The colonies of these two kinds of bacteria were more than other strains in a single sample.鲎 test and found that: 泔 water oil bacterial metabolites - lipopolysaccharide content of 100-1000 times higher than normal cooking oil. CONCLUSION: The dominant bacteria in the swill were identified as Klebsiella pneumoniae and Escherichia coli. Bacterial metabolites - lipopolysaccharide can be used as a target for the detection of swill oil antigen.