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为了揭示耐热粳稻资源热粳35的遗传特性,以热粳35/协B F2群体为研究材料,构建了包含140个SSR标记的分子图谱;借助构图分子数据分析标记基因型的分离情况,并进行耐热性QTL分析。结果表明,该图谱覆盖基因组全长2 157.7 c M,标记间平均图距15.4 c M,45个(28.5%)标记极显著偏向亲本或杂合子(P<0.01)。在第1、第3、第4、第6、第7和第8号染色体上发现9个偏分离热点区域。其中,有4个偏分离热点区域可能与孢子体或配子体选择有关,在第4、第8和第12号染色体上共检测到3个耐热性QTL。本研究结果为耐热粳稻资源的遗传机理解析和新品种选育提供了参考信息。
In order to reveal the genetic characteristics of heat-tolerant Japonica rice, Hot Japonica 35, a molecular map containing 140 SSR markers was constructed based on the BJ population of Hot Japonica 35 / Xie. Segregation of marker genotypes was analyzed by means of compositional molecular data. Heat tolerance QTL analysis. The results showed that the map covered a total length of 2 157.7 cM and the average distance between markers was 15.4 cM. 45 (28.5%) markers showed extremely significant bias towards parents or heterozygotes (P <0.01). Nine partial hotspot regions were found on chromosomes 1, 3, 4, 6, 7 and 8. Among them, four hot spots with partial segregation may be related to sporophyte or gametophyte selection. Three heat-resistant QTLs were detected on chromosomes 4, 8 and 12. The results of this study provide reference information for genetic mechanism analysis and selection of new varieties of heat-tolerant japonica rice.