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目的探讨慢性丙型肝炎(HCV)患者CD4+CD25+Treg细胞对外周血树突状细胞的作用。方法对35例HCV患者和35例健康对照各抽取外周血,采用密度梯度离心法分离外周血单个核细胞(PBMC),采用特异性免疫磁珠分选获得CD4+CD25+Treg细胞,并体外诱导培养获得树突状细胞(DCs);将CD4+CD25+Treg细胞与DC共培养5d后,采用流式细胞仪检测DC表面标志CD83、CD80、HLADR的表达,同时酶联免疫吸附法检测上清液中IL-10和TGF-β含量。结果与健康对照组相比,HCV患者CD83、CD80和HLA-DR的表达均显著下降,差异有统计学意义(P<0.01);HCV组患者CD4+CD25+Treg分泌IL-10和TGF-β的水平均高于健康组(P<0.01)。结论 HCV患者外周血Treg细胞能够抑制DC的成熟,细胞因子参与了免疫应答的调节。
Objective To investigate the effect of CD4 + CD25 + Treg cells on peripheral blood dendritic cells in patients with chronic hepatitis C (HCV). Methods Peripheral blood was collected from 35 HCV patients and 35 healthy controls. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation. CD4 + CD25 + Treg cells were sorted by specific immunomagnetic beads and induced in vitro The DCs were cultured and cultured. After co-cultured with CD4 + CD25 + Treg cells for 5 days, the expression of CD83, CD80 and HLADR on DCs were detected by flow cytometry. The supernatants were detected by enzyme-linked immunosorbent assay Fluid IL-10 and TGF-β content. Results The expression of CD83, CD80 and HLA-DR in HCV patients was significantly lower than that in healthy controls (P <0.01). The levels of IL-10 and TGF-β secreted by CD4 + CD25 + Treg in HCV patients Higher than the healthy group (P <0.01). Conclusion Treg cells from peripheral blood of patients with HCV can inhibit the maturation of DC. Cytokines are involved in the regulation of immune response.