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目的:探讨在缺氧性损害下肼屈嗪对视网膜色素上皮(ARPE-19)细胞的抗氧化效果以及活性氧(ROS)在此效果中的作用。方法:用人视网膜色素上皮细胞研究肼屈嗪对氧化应激的作用,包括特丁基氢过氧化物(t-BHP)、过氧化氢(H2O2)、叠氮化钠(NaN3),以及缺氧引起的细胞坏死。用MTT检验测试细胞活性。结果:用ROS诱导的氧化应激治疗ARPE-19细胞,肼屈嗪在抵抗t-BHP、H2O2、缺氧引起的细胞坏死中表现出浓度依赖性,但对NaN3不具有浓度依赖性。这一作用中不涉及到一氧化氮(NO)。结论:肼屈嗪在ARPE-19细胞中表现出抗氧化应激诱导的破坏,这一作用可能是因为对ROS的净化剂作用。所以肼屈嗪可能用于治疗老年黄斑变性。
OBJECTIVE: To investigate the antioxidative effect of hydralazine on retinal pigment epithelium (ARPE-19) cells under hypoxic conditions and the role of reactive oxygen species (ROS) in this effect. Methods: The effects of hydralazine on oxidative stress were studied using human retinal pigment epithelial cells, including t-BHP, H2O2, NaN3 and hypoxia Cell necrosis. Cell viability was tested by MTT assay. RESULTS: ARPE-19 cells were treated with ROS-induced oxidative stress. Hydralazine showed concentration-dependent inhibition of t-BHP, H2O2 and hypoxia-induced cell necrosis, but not concentration-dependent of NaN3. Nitric oxide (NO) is not involved in this role. CONCLUSIONS: Hydralazine exhibited anti-oxidative stress-induced destruction in ARPE-19 cells, a possible role for scavenger of ROS. Hydralazine may therefore be used to treat age-related macular degeneration.