Construction of the Subtracted cDNA Library of Striatal Neurons Treated with Long-term Morphine

• 作 者: Bo Bai Hai-qing Liu Jing Chen
• 机 构: DepartmentofNeurobiology,DepartmentofPhysiology
• 出 处: 中国医学科学杂志：英文版
• 发表日期: 2011年1期
• 关键词: cDNA文库 吗啡耐受 神经元 纹状体 治疗 CAMP反应元件结合蛋白 差异表达基因 mRNA表达 neuron morphine tolerance supp
• 基金项目: Supported by the National Natural Science Foundation of China （81070961,30770676, and 30870932）, the Natural Science Foundation of Shandong Province （ZR2009DZ004）, and the Science and Technology Burea

Objective To construct a morphine tolerance model in primarily cultured striatal neurons,and screen the differentially expressed genes in this model using suppression subtractive hybridization (SSH).Methods Sbtracted cDNA libraries were constructed using