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Division of Reproductive Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China *DEPT. Of Pharmacology, Shanghai Tiedao University, Medical College Shanghai 200070, China By using the morphology and the viability of cells index, the direct effects of anordrin on serum free primary cultures of rat luteal cells, human decidual cells and trophoblast cells were observed.Meanwhile, the effect of anordrin on the secretive function of rat luteal cells was also observed. The results indicated that (1) anordrin has damaging effects on rat luteal cells, human decidual cells and trophoblast cells. The LD 50 s were 14.34±0.9 μg/ml, 17.33±4.1 μg/ml and 34.87±4.9 μg/ml respectively. (2) With nonlethal dose (5 μg/ml), the activity of progesterone secretion of rat lutein cells which was stimulated by hCG and pregnenolone was not influenced by anordrin while the stimulating activity of forskolin was inhibited remarkably.The results suggest that luteolytic action is the main mechanism of the termination of early pregnancy by anordrin and the direct damaging effects of anordrin on decidua and cytotrophoblasts also play a role in the termination of early pregnancy.
Division of Reproductive Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China * DEPT. Of Pharmacology, Shanghai Tiedao University, Medical College Shanghai 200070, China By using the morphology and the viability of cells index, the direct effects of anordrin on serum free primary cultures of rat luteal cells, human decidual cells and trophoblast cells were observed. However, the effect of anordrin on the secretive function of rat luteal cells was also observed. The results indicated that (1) anordrin has damaging effects (2 μg) with nonlethal dose (5 μg / ml) on rat luteal cells, human decidual cells and trophoblast cells. The LD 50 s were 14.34 ± 0.9 μg / ml, 17.33 ± 4.1 μg / ml and 34.87 ± 4.9 μg / ), the activity of progesterone secretion of rat lutein cells which was stimulated by hCG and pregnenolone was not influenced by anordrin while the stimulating activity of forskolin was inhibited remax rkably.The results suggest that luteolytic action is the main mechanism of the termination of early pregnancy by anordrin and the direct damaging effects of anordrin on decidua and cytotrophoblasts also play a role in the termination of early pregnancy.