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目的探讨体外培养成熟的人类卵子冻存的可行性。方法中山大学附属第一医院从2003年3月至2003年9月在ICSI治疗周期中共收集到101个GV期卵子和53个MI期卵子,根据卵子发育阶段不同将不成熟卵子分为GV期组和MI期组,体外培养成熟后应用慢速冷冻方法进行卵子冷冻,解冻后用ICSI受精并进行胚胎体外培养,分别比较GV期组和MI期组卵子的复苏率、受精率、优质胚胎率和囊胚发育情况。结果经体外培养后GV期组和MI期组分别有64个和44个卵子成熟,GV期组卵子体外成熟率为634%(64/101),显著低于MI期组的830%(44/53)(P<005),冷冻后复苏率分别为719%(46/64)和818%(36/44),受精率分别为609%(28/46)和639%(23/36),优质胚胎率分别为536%(15/28)和652%(15/23),在统计学上差异均无显著性意义。结论在ICSI治疗周期中的GV期和MI期的卵子经体外成熟后可以成功地进行冷冻保存。
Objective To investigate the feasibility of culturing mature human eggs in vitro. Methods A total of 101 GV and 53 MI eggs were collected from the First Affiliated Hospital of Sun Yat-sen University from March 2003 to September 2003 during ICSI treatment. Immature eggs were divided into GV group And MI group. After being cultured in vitro, the cells were frozen in the way of slow freezing and thawed. After thawing, the cells were fertilized with ICSI and cultured in vitro. The recovery rate, fertilization rate, Blastocyst development. Results After in vitro culture, 64 and 44 eggs were matured in GV group and MI group, respectively. The in vitro maturation rate of egg in GV group was 634% (64/101), significantly lower than that in MI group (830%, 44 / (P <0.05). The rates of resuscitation after cryopreservation were 719% (46/64) and 818% (36/44), respectively. The fertilization rates were 609% (28/46) and 639% High-quality embryos were 536% (15/28) and 652% (15/23) respectively, with no statistically significant difference. Conclusion The oocytes in GV phase and MI phase in ICSI treatment cycle can be successfully cryopreserved after in vitro maturation.