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目的:探讨p63及其亚型△Np63、TAp63在正常胎鼠和腭裂模型胎鼠侧腭突上皮,特别是在胚胎腭中线上皮(medial edge epithelial,MEE)/胚胎腭中线上皮带(medial epithelial seam,MES)中的表达及变化情况,阐明p63及其亚型△Np63、TAp63在MEE/MES消失过程中的作用。方法:建立全反式维A酸(all-trans retinoic acid,atRA)诱导的胎鼠腭裂模型,实验组在鼠孕期第10天(gestation day,GD10)按100 mg/kg给予atRA灌胃,对照组则给予同等剂量的玉米油,分别在GD13.5、GD14.5、GD15.5、GD16.5解剖取得胎鼠的腭突,通过HE染色、扫描电镜进行形态学观察,采用免疫组化方法检测胎鼠侧腭突上皮p63、△Np63及TAp63的表达。结果:在GD14.5,对照组p63和△Np63在侧腭突MEE细胞内基本呈阴性表达,而实验组则呈阳性表达。在GD15.5,对照组MEE/MES消失,腭突基本融合,侧腭突所有被覆上皮基底层细胞再次出现p63和△Np63阳性表达;实验组MEE/MES则未完全消退,p63和△Np63在消退异常的MEE细胞内仍呈阳性表达。在GD16.5对照组,腭突融合完成,侧腭突被覆上皮基底层细胞p63和△Np63仍呈阳性表达;而实验组未融合,呈腭裂,p63和△Np63在MEE细胞内呈阴性表达。结论:在胎鼠侧腭突上皮发育过程中,存在p63及其亚型△Np63特定时空的差异表达,提示△Np63参与侧腭突上皮的分化调控,尤其是参与了MEE/MES消失过程中的信号调控。
Objective: To investigate the expression of p63 and its subtypes △ Np63 and TAp63 in the palatal epithelium of normal fetus and palatal palate, especially in the medial edge epithelial (MEE) / medial epithelial seam , MES) and clarify the role of p63 and its subtypes △ Np63, TAp63 in the disappearance of MEE / MES. Methods: The model of fetal rat cleft palate induced by all-trans retinoic acid (atRA) was established. The experimental group was given 100 mg / kg gestation day (GD10) The same dose of corn oil was given to the rats. The palatal dentate of fetus was obtained by dissection in GD13.5, GD14.5, GD15.5 and GD16.5 respectively. Morphology was observed by HE staining and scanning electron microscopy. Immunohistochemistry The expression of p63, △ Np63 and TAp63 in fetal rat palatal epithelium was detected. Results: At GD14.5, p63 and △ Np63 in control group were almost negative in MEE cells, while those in experimental group were positive. At GD15.5, the MEE / MES disappeared and the palatal fossa basically fused. The expression of p63 and △ Np63 in all the epithelial basal cells of the palatal palate again appeared. The MEE / MES in the experimental group did not completely disappear. The expression of p63 and △ Np63 in the Abnormal MEE cells were still positive expression. In the GD16.5 control group, palatal fossa fusion was completed. The p63 and △ Np63 in epithelial basal lamina of the palatal palate were still positive. In the experimental group, cleft palate, p63 and △ Np63 were negatively expressed in MEE cells. Conclusion: The differential expression of p63 and its specific subunit △ Np63 in the development of fetal palatal epithelium suggests that △ Np63 is involved in the differentiation regulation of palatal palatal epithelium, especially involved in the disappearance of MEE / MES Signal conditioning.