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目的:研究银杏叶提取物(GbE)对CCl4肝纤维化大鼠肝细胞凋亡的影响,探讨银杏叶提取物抗肝纤维化的作用机制。方法:采用CCl4建立雄性SD大鼠肝纤维化动物模型,同时以不同剂量的GbE灌胃6周进行干预;光镜观察肝组织的病理学变化,TUNEL法检测肝细胞的凋亡情况,免疫组化法检测凋亡相关蛋白Bcl-2、Bax的表达。结果:模型组大鼠肝组织存在不同程度的炎症和纤维化,肝细胞的凋亡指数和Bax表达较正常组明显增高(P<0.01)、Bcl-2表达则较正常组明显减少(P<0.01);不同剂量的GbE能减轻CCl4肝纤维化大鼠肝组织的炎症和纤维化程度(P<0.01、P<0.05),促进肝组织中Bcl-2表达的同时抑制Bax的表达(P<0.01、P<0.05),减少肝细胞的凋亡(P<0.01)。结论:银杏叶提取物的抗纤维化作用可能是通过调节Bcl-2和Bax的表达,减少肝细胞的凋亡来实现的。
Objective: To study the effect of Ginkgo biloba extract (GbE) on hepatocellular apoptosis in CCl4-induced liver fibrosis in rats and to explore the mechanism of the effect of Ginkgo biloba extract on hepatic fibrosis. Methods: The animal model of hepatic fibrosis in male Sprague-Dawley rats was established by CCl4. At the same time, different doses of GbE were administered intragastrically for 6 weeks for intervention. The pathological changes of liver tissues were observed under light microscope. The apoptosis of hepatocytes was detected by TUNEL. Apoptosis-related proteins Bcl-2, Bax were detected by immunohistochemistry. Results: The liver tissue of rats in model group had different degree of inflammation and fibrosis. The apoptosis index and Bax expression of hepatocytes in model group were significantly higher than those in normal group (P <0.01), while the expression of Bcl-2 was significantly lower than that in normal group (P < 0.01). Different doses of GbE could reduce the degree of inflammation and fibrosis in hepatic tissue of CCl4-induced hepatic fibrosis rats (P <0.01, P <0.05), and promote the expression of Bcl-2 and Bax in liver tissues (P < 0.01, P <0.05), and decreased the apoptosis of hepatocytes (P <0.01). Conclusion: The anti-fibrosis effect of Ginkgo biloba extract may be through regulating the expression of Bcl-2 and Bax and reducing the apoptosis of hepatocytes.