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目的从富含多糖和小分子杂质的赤芍干叶片中快速分离出适用于各生物技术分析的高质量基因组DNA,为赤芍的鉴定研究提供实验基础。方法以常规的SDS法为基础,进一步优化DNA提取各步骤,获得了一种快速可行的提取高质量赤芍干叶片DNA的方法。结果使用该方法从4个不同产地的赤芍干叶片中分离的DNAA260/A280分别为1.90、1.88、1.91、1.93,所需时间均小于2.5 h,所得DNA分别直接用于PCR分析,结果满意。结论该方法用于提取赤芍干叶片DNA快速可行,可有效地从富含多糖和小分子杂质的组织中分离出高质量DNA,方法简便、快速,成本低。
Objective To rapidly isolate high-quality genomic DNA suitable for various biotechnological analyses from the leaves of P. erythropolis that are rich in polysaccharides and small-molecule impurities, and to provide experimental basis for the identification and identification of Radix Paeoniae Rubra. Methods Based on the conventional SDS method, the steps of DNA extraction were further optimized, and a rapid and feasible method for extracting high quality DNA from dry leaves of A. perfoliatum was obtained. Results The DNAA260/A280 isolated from leaves of four different habitats were 1.90, 1.88, 1.91, and 1.93, respectively. The time required was less than 2.5 hours. The obtained DNA was directly used for PCR analysis. The results were satisfactory. Conclusion The method is rapid and feasible for the extraction of DNA from dry leaves of Blattella japonica. It can effectively separate high-quality DNA from tissues rich in polysaccharides and small molecular impurities. The method is simple, rapid, and low in cost.