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多数化学致癌物经细胞色素(Cyt)P-450、特别是经3-甲基胆蒽(MC)诱导的P-448同工酶代谢活化后,表现出致癌和致突变活性。为了阐明在致突变试验中使用富含Cyt P-448L或cyt P-448 H/L的微粒体,可否用于确定P-448同工酶对致癌物所起的致突变活化作用。作者用不同诱导物制备的大鼠肝微粒体测试了九种致癌性芳香胺和B(a)P的致突变活化作用。富含Cyt P-448H/L和P-448 L微粒体的制备:给7周龄的SD大鼠腹腔注射0.11
Most chemical carcinogens exhibit oncogenic and mutagenic activity after being metabolically activated by P-448 isoenzyme, which is induced by cytochrome (Cyt) P-450, especially by 3-methylcholanthrene (MC). Helicobacter pylori antibody was used to determine the mutagenic activation of P-448 isoenzyme in carcinogenesis in order to elucidate the use of microsomes enriched for Cyt P-448L or cyt P-448 H / L in mutagenic assays. The authors tested rat liver microsomes prepared with different inducers for mutagenic activation of nine oncogenic aromatic amines and B (a) P. Preparation of Cyt P-448H / L and P-448 L-rich microsomes: Seven-week-old SD rats were intraperitoneally injected with 0.11