论文部分内容阅读
为保持食用菌菌种的优良种性和防止杂菌污染,较长期保藏斜面菌种,我们试用蜡封管口法,效果良好。采用此法无需专门设备、特殊药物,材料易得,操作简便。具体做法如下: 1.培养基的制备。取大型试管(口径为20～25毫米),适当加大培养基中琼脂的比例(占3％),增加注入试管中培养基的数量(占容积的1/4),棉塞要做紧。培养基的配方应根据该菌种生长发育对营养的需求,选择最佳方案,一般多采用马铃薯葡萄糖琼脂培养基(PDA培养基)。 2.接种培养。在已选定的菌株中,挑取茁壮的菌丝为接种体,接种体不要过大(3×3毫米)。在该菌生长最适的温度下进行培养,长满斜面后须继续培养2～3天。 3.剪塞浸蜡。将已培养好的斜面,齐管
In order to maintain the excellent species of edible fungi and prevent the bacterial contamination, the long-term preservation of bevel bacteria, we try wax seal nozzle method, the effect is good. Using this method without special equipment, special drugs, materials, easy to get, easy to operate. The specific practices are as follows: 1. Preparation of medium. Take a large test tube (diameter of 20 to 25 mm), appropriate to increase the proportion of agar medium (3%), increase the amount of medium injected into the test tube (accounting for the volume of 1/4), tampon to do tight. Medium formula should be based on the species growth and development of the nutritional needs, choose the best solution, the general use of potato dextrose agar (PDA medium). Inoculation training. In the selected strains, pick strong mycelium as inoculum, inoculum should not be too large (3 × 3 mm). In the optimum temperature for the growth of the bacteria culture, overgrown to continue training after 2 to 3 days. 3. Cut plug dip wax. Will have cultivated a good slope, Qi tube