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多胺化合物,包括腐胺、亚精胺和精胺,存在于所有哺乳动物组织和体液中。在人精浆中,精胺(spermine)浓度甚高(2—15mmol/l)。然而精胺在生殖过程中的功能尚不清楚。有关精胺对哺乳动物精子活力、代谢和受精作用的报道也存在诸多矛盾。近几年来,一些作者证明精胺可抑制顶体素酶原的转化,导致受精率下降。此外,精胺还可增加人精子cAMP水平。Stanger和Quinn观察到精胺不仅可提高小鼠体外受精率而且还可缩短受精时间;但我们以往观察到精胺抑制豚鼠精子体外获能,导致受精率下降。这些结果表明,精胺可能参与精子获能和受精过程。本文研究精胺对地鼠精子体外获能和受精的作用。
Polyamine compounds, including putrescine, spermidine and spermine, are found in all mammalian tissues and body fluids. In human seminal plasma, spermine concentration is very high (2-15 mmol / l). However, the function of spermine in the reproductive process is not clear. There are also many contradictions about the effect of spermine on mammalian sperm motility, metabolism and fertilization. In recent years, some authors have shown that spermine inhibits the conversion of apokonin, leading to a decline in fertilization rates. In addition, spermine can increase cAMP levels in human sperm. Stanger and Quinn observed that spermine not only increased in vitro fertilization rates in mice but also shortened fertilization time; however, we previously observed that spermine inhibits in vitro capacitation of guinea pig sperm and results in decreased fertilization rates. These results suggest that spermine may be involved in sperm capacitation and fertilization. This article studies spermine on gopher spermatozoa in vitro capacitation and fertilization.