论文部分内容阅读
目的研究一株耐碱链霉菌(Streptomyces pleomorphus sp. nov.)发酵液乙酸乙酯提取物的化学成分。方法采用硅胶柱色谱、凝胶柱色谱方法进行分离纯化,利用波谱分析法结合理化性质确定化合物结构。结果从S.pleomorphus sp. nov.发酵液的乙酸乙酯萃取物中分离得到9个已知化合物,分别鉴定为路易斯安那碱A(1)、路易斯安那碱B(2)、4′,7-二羟基异黄酮(3)、4′,7-二羟基-6-甲氧基异黄酮(4)、4′,5,7-三羟基异黄酮(5)、14-甲基十五烷酸(6)、(E)-3-甲硫基-丙烯酸(7)、4-羟基苯乙醇(8)、β-谷甾醇(9)。结论9个化合物均为首次从S.pleomorphus sp. nov.代谢产物中分离得到。
Objective To study the chemical constituents of ethyl acetate extract from a fermentation broth of Streptomyces pleomorphus sp. Nov. Methods The compounds were isolated and purified by silica gel column chromatography and gel column chromatography. Their chemical structures were determined by spectral analysis. Results Nine known compounds were isolated from the ethyl acetate extract of S.pleomorphus sp. Nov. Fermentation broth and identified as Louisiana base A (1), Louisiana base B (2), 4 ’ 7-dihydroxy-6-methoxyisoflavone (4), 4 ’, 5,7-trihydroxy isoflavone (5), 14- (6), (E) -3-methylthio-acrylic acid (7), 4-hydroxyphenethyl alcohol (8) and β-sitosterol (9). Conclusion All nine compounds were isolated from S.pleomorphus sp. Nov. Metabolites for the first time.