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通过对甜菜坏死黄脉病毒提纯液进行紫外扫描,获得了典型的核蛋白曲线,OD280/OD260=0.901,推算病毒液浓度约为0.58mg/ml。采用SDS-PAGE测定证实BNYVV只有1种蛋白亚基,分子量为20749道尔顿。
A typical nuclear protein curve was obtained by UV scanning of the beet necrotic vein virus, OD280 / OD260 = 0.901, and the estimated virus concentration was about 0.58mg / ml. SDS-PAGE assay confirmed that BNYVV has only one protein subunit with a molecular weight of 20749 Daltons.