L-Plastin deficiency produces increased trabecular bone due to attenuation of sealing ring formation

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INTRODUCTIONrnOsteoclasts are multinucleated, terminally differentiated giant cells, originating from the fusion of monocytes, and are involved in bone resorption. During the adhesion of osteoclasts to the bone during resorption, an actin-rich ring-like sealing zone (SZ) forms, providing a tight attachment area to the bone surface and circumscribes the area of bone resorption. The molecular dynamics of bone resorption and the cytoskeletal changes needed to carry out the formation of resorptive structures is complex and is fertile ground for therapeutic interventions to regulate bone-resorbing activities. Sealing ring formation is a hallmark of osteoclast activation for bone resorption.1-5 Sealing zones are delineated as ring-shaped structures enriched in bundles of actin. Formation of actin rings defines the functionality of an osteoclast plated on mineralized matrix, dentine, or histological bone slices in vitro. In resorbing osteoclasts, rings give rise to SZs by growing individually and making a thicker and more central and stable super-ring.6 Researchers termed these structures as SZ rings, actin rings, or SZs.7-15 The SZ defines the resorption area of the bone, consisting of a dynamic actin-rich ring-like structure which we have designated as a sealing ring since 2007.1-2,16-20
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