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目的 研究流行于河南省献血员中的人免疫缺陷病毒（ＨＩＶ） 外膜蛋白（ｅｎｖ） 基因亚型。方法 提取ＨＩＶ 感染者外周血单个核白细胞（ＰＢＭＣ） 的ＤＮＡ，经套式聚合酶链反应（ＮｅｓｔｅｄＰＣＲ） 扩增ｅｎｖ 基因片断，对Ｃ２Ｖ３ 区３５０ ～４５０ 核苷酸顺序进行测定和分析。结果 ２０ 份样品的ｅｎｖ 基因序列与Ｂ亚型最为接近，基因离散率为８ ．３４ ％ ，与其它国际亚型的基因离散率在２０ ％ 以上；与流行于云南的Ｂ 亚型毒株基因离散率为４ ．１８ ％ ；样品间的基因离散率为３ ．４ ％ 。结论 流行于河南省献血员中的ＨＩＶ 属Ｂ亚型，并与云南的流行株密切相关；其在河南的流行时间在３ ～６ 年间。
Objective To study the human immunodeficiency virus (HIV) outer membrane protein (env) gene subtype that is popular among blood donors in Henan Province. Methods The DNA of peripheral blood mononuclear leucocytes (PBMC) from HIV-infected individuals was extracted and the env gene fragment was amplified by NestedPCR. The nucleotide sequence of 350 to 450 nucleotides in C2V3 region was determined. analysis. Results The env gene sequence of 20 samples was the closest to that of subtype B, with a genetic divergence rate of 8. 34%, with other international subtypes of gene dispersion rate of more than 20%; and strains of subtype B of Yunnan, the gene dispersion rate of 4. 18%; the gene dispersion rate between samples is 3. 4%. Conclusion The HIV prevalence among blood donors in Henan Province is subtype B, which is closely related to the endemic strains in Yunnan Province. The epidemic time in Henan Province is between 3 and 6 years.