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胸腺抑肽是从动物胸腺中提取的具有免疫抑制作用的多肽,国内报道较少。本文观察了猪胸腺抑肽对小鼠免疫功能的调节作用。1 材料和方法1.1 材料 猪胸腺抑肽由南京大学生物化学系金以丰教授制备,0.47mg/ml,分子量12000。BCG50mg/支,为卫生部上海生物制品研究所产品。1.2 动物 Balb/c,小鼠20只,8～12周龄,20g,雌性,江苏省实验动物中心提供。1.3 实验分组及处理 小鼠随机分为4组,每组5只,尾静脉注射胸腺抑肽0.1mg/d,分别连续注射0、5、10和15d。注射结束的第2天进行免疫学测定。1.4 循环免疫复合物(CIC)测定 按文献进行。1.5 胸腺细胞对ConA刺激的增殖反应测定 见文献。
Thymosin is extracted from the animal thymus immunosuppressive peptides, less reported in the country. In this paper, we observed the regulatory effect of porcine thymosin on immune function in mice. 1 Materials and methods 1.1 Materials Pig thymosin by the Department of Biochemistry, Nanjing University Professor Jin Yifeng preparation, 0.47mg / ml, molecular weight 12,000. BCG50mg / support, the Ministry of Health Shanghai Institute of Biological Products products. 1.2 Animals Balb / c, 20 mice, 8-12 weeks old, 20g, female, provided by Experimental Animal Center of Jiangsu Province. 1.3 Experimental grouping and treatment Mice were randomly divided into 4 groups of 5, each group was injected thymosin 0.1mg / d into the tail vein for 0, 5, 10 and 15 days respectively. Immunoassay was performed on the second day after injection. 1.4 Determination of circulating immune complexes (CIC) according to the literature. 1.5 Thymocyte proliferation assay ConA stimulated see literature.