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目的:探讨性激素和活性氧协同作用对肝癌细胞增殖的影响。方法:采用MTT法观察性激素(雌二醇或睾酮)和H2O2单独处理或同时处理表达性激素受体的Hepa1-6细胞的增殖率。结果:浓度<10μmol/L的雌二醇或睾酮对细胞的增殖无显著影响。H2O2浓度为1～15μmol/L时,促进细胞增殖。>100μmol/L则抑制细胞的增殖甚至诱导细胞的凋亡和坏死。雌激素或雄激素与15μmol/LH2O2联合使用时,细胞增殖消失;与200μmol/LH2O2联合使用时,增强了H2O2对细胞的毒性作用。性激素可抑制低浓度H2O2诱导的细胞增殖效应并可加强中等浓度H2O2的细胞损伤作用。结论:性激素与活性氧的相互作用抑制了细胞的增殖,为进一步探讨性激素与活性氧的相互作用关系提供了实验依据,为性激素依赖或相关性肿瘤的治疗提供了新的思路。
Objective: To explore the synergistic effects of sex hormones and reactive oxygen species on the proliferation of hepatoma cells. Methods: MTT assay was used to observe the proliferation rate of Hepa 1-6 cells expressing sex hormones by sex hormones (estradiol or testosterone) and H2O2 alone or in combination. RESULTS: Estradiol or testosterone at concentrations <10 μmol / L had no significant effect on cell proliferation. H2O2 concentration of 1 ~ 15μmol / L, promote cell proliferation. > 100μmol / L inhibits cell proliferation and even induces cell apoptosis and necrosis. When the combination of estrogen and androgen with 15μmol / LH 2 O 2 disappeared, the cell proliferation was enhanced when combined with 200μmol / LH 2 O 2. Sex hormones can inhibit the cell proliferation induced by low concentration of H2O2 and can enhance the cell injury of moderate concentration of H2O2. Conclusion: The interaction between sex hormones and reactive oxygen species inhibits the proliferation of cells, providing experimental basis for further exploring the interaction between sex hormones and reactive oxygen species, providing a new idea for the treatment of sex hormone dependent or related tumors.