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用顺行演变与免疫组织化学结合的双标技术，电镜下观察大鼠孤束核内５－羟色胺样免疫反应（５－ＨＴ－ＬＩ）轴突终末的突触联系，特别是与迷走神经初级传入（溃变）终末的关系。发现：１．５－ＨＴ－ＬＩ轴突终末和迷走神经初级传入终末终止于同一个树突形成轴－树突触；２．５－ＨＴ－ＬＩ轴突终末与树突形成轴－树突触；３．５－ＨＴ－ＬＩ轴突终末与迷走神经初级传入终末或非标记的轴突终未形成轴一轴相贴（ａｘｏ－ａｘｏｎｉｃ－ｃｏｎｔａｃｔ）。以上结果提示，孤束核内的５－ＨＴ能神经成分可能通过突触后、突触前机制调控经迷走神经传入的信息。
Synaptic connections of 5-hydroxytryptamine-like immunoreactivity (5-HT-LI) axon termini in the nucleus tractus solitarius were observed by electron microscopy using a double-stranded technique combined with immunohistochemistry. Incoming (ulceration) the relationship between the end. It was found that both the 1.5-HT-LI axon terminals and the vagal primary afferent terminals terminate in the same dendrite to form axonal-dendritic synapses; Dendrites. The axo-axonic contact was not formed at the end of 3.5-HT-LI axon and the terminal of primary vaginal afferent end or non-labeled axon. The above results suggest that 5-HT neurons in the nucleus tractus soliton may regulate the afferent information via the vagus nerve via the postsynaptic and presynaptic mechanisms.