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输卵管和/或卵巢移植可用于治疗下列不孕症病人:①先天性附件异常或附件切除;②通过输卵管整形术,输卵管功能仍未恢复;③体外受精失败。为研究该法的可用性,本文报道使用小白鼠试验行显微外科全血管附件同源移植和异体移植。选择未交配过的成熟Lewis和BN雌性白鼠,重量175～200g。供鼠用3 ml肝素化盐水(200U/ml)抗动物凝血后,分别在右侧卵巢动、静脉起始部上、下方扎紧下腔静脉和主动脉,然后在子宫右侧角远端切断处的上下扎紧右侧子宫血管,切除或切断结扎线间的血管。切除右侧卵管、卵巢及远侧子宫角。伴有血供的移植物置于受鼠腹腔内适当位置,供鼠主动脉、下腔静脉与受鼠主动脉、下腔
Tubal and / or ovarian transplantation can be used to treat the following infertility patients: ① congenital anomalies or attachment removal; ② by tubal surgery, tubal function has not been restored; ③ failure of in vitro fertilization. In order to investigate the availability of this method, this paper reports the use of microsurgery whole-vessel accessory homologous and allograft transplantation in mice. Select unmatched mature Lewis and BN female mice, weight 175 ~ 200g. For mice with 3 ml of heparinized saline (200U / ml) anti-coagulation of animals, respectively, in the right ovary, the starting part of the vein, the lower part of the lower vena cava and aortic tension, and then cut off in the right corner of the uterus Tight up and down tight at the right uterine vessels, resection or cut off the blood vessels between the ligature. Excision of the right oviduct, ovary and distal uterine horn. Accompanied by a blood supply of the graft placed in the appropriate position in the abdominal cavity of the mouse for the rat aorta, inferior vena cava and the rat aorta, lower chamber