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目的测定大鼠胃粘膜氨基己糖和磷脂含量，以证实其对评价胃粘膜防御机制的价值．方法大鼠３６只分为５个组．Ⅰ组为正常对照组；Ⅱ组为无水乙醇损伤对照组；Ⅲ组为胶体次枸橼酸铋加无水乙醇组；Ⅳ组为硫糖铝加无水乙醇组；Ⅴ组为雷尼替丁加无水乙醇组．测定胃粘膜氨基己糖、磷脂含量以及胃粘膜血流（ＧＭＢＦ）、跨胃粘膜电位（ＰＤ）及胃粘膜表面疏水性．结果乙醇损伤大鼠胃粘膜后，氨基己糖（ｍｇ／ｇ蛋白：０２１±００３ｖｓ０５９±００４）和磷脂含量（ｍｇ／ｇ蛋白：０３２±００４ｖｓ０６４±００４）显著下降（Ｐ＜００１）．胶体次枸橼酸铋和硫糖铝可使氨基己糖及磷脂较乙醇损伤对照组显著升高（Ｐ＜００１）．胃粘膜氨基己糖及磷脂含量与ＧＭＢＦ，ＰＤ和粘膜表面疏水性呈显著正相关．结论胃粘膜氨基己糖及磷脂含量对评价胃粘膜损伤和药物疗效有重要价值．
Objective To determine the content of aminohexose and phospholipids in rat gastric mucosa to confirm its value in evaluating the gastric mucosal defense mechanism. Methods Thirty-six rats were divided into five groups. Group Ⅰ was normal control group, group Ⅱ was ethanol control group, group Ⅲ was colloid bismuth citrate plus ethanol group, group Ⅳ was sucralfate plus ethanol group, groupⅤ was ranitidine Ding added anhydrous ethanol group. Determination of gastric mucosal hexosamine, phospholipid content and gastric mucosal blood flow (GMBF), trans-gastric mucosal potential (PD) and gastric mucosal surface hydrophobicity. Results After ethanol injury in gastric mucosa, the concentrations of hexosamine (mg / g protein: 021 ± 003vs059 ± 004) and phospholipid (mg / g protein: 032 ± 004vs064 ± 004) decreased significantly (P <001). Colloidal bismuth citrate and sucralfate significantly increased levels of hexosamine and phospholipids compared with ethanol-treated controls (P <001). Gastric mucosal hexosamine and phospholipid content and GMBF, PD and mucosal surface hydrophobicity was significantly positively correlated. Conclusion Gastric mucosa hexosamine and phospholipid content of gastric mucosal injury evaluation and drug efficacy has important value.