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取昆明种新生小鼠后肢肌肉1 mm~3置于普通培养皿内,在含羊成骨蛋白(sBMP)1、5、10、50 μg/ml的1640培养基内培养10 d,sBMP各浓度组均有成软骨反应,10μg/ml组成骨反应最好,成骨率达80％,有4个肌肉块完全转化成软骨团。本实验建立了一个体外诱导成骨的实验模型,为骨诱导的深入研究提供了一个简便的组织培养方法。
The hindlimb muscles of Kunming newborn mice were placed in ordinary petri dishes 1 mm ~ 3 and cultured in 1640 medium containing 1, 5, 10 and 50 μg / ml sBMP for 10 d. The concentrations of sBMP The cartilage reaction was observed in all groups. The best 10μg / ml bone formation reaction resulted in an 80% osteogenic rate. Four muscle blocks were completely transformed into cartilage mass. This experiment established an experimental model of in vitro osteogenesis, which provided a convenient tissue culture method for in-depth study of osteoinduction.