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AIM:To determine whether cyclooxygenase-2(COX-2)wasexpressed in human esophageal squamous cell carcinoma.METHODS:Quantitative reverse transcription-polymerasechain reaction(RT-PCR),western blotting,immunohistoc-hemistry and immunofluorescence were used to assessthe expression level of COX-2 in esophageal tissue.RESULTS:COX-2 mRNA levels were increased by>80-foldin esophageal squamous cell carcinoma when comparedto adjacent noncancerous tissue.COX-2 protein waspresent in 21 of 30 cases of esophageal squamous cellcarcinoma tissues,but was undetectable in noncanceroustissue.Immunohistochemistry was performed to directlyshow expression of COX-2 in tumor tissue.CONCLUSION:These results suggest that COX-2 may bean important factor for esophageal cancer and inhibitionof COX-2 may be helpful for prevention and possiblytreatment of this cancer.
AIM: To determine whether cyclooxygenase-2 (COX-2) wasexpressed in human esophageal squamous cell carcinoma. METHODS: Quantitative reverse transcription-polymerase chain reaction (RT- PCR), western blotting, immunohistoc-hemistry and immunofluorescence were used to assessthe expression level of COX-2 protein waspresent in 21 of 30 cases of esophageal squamous cell carcinoma tissues, but was undetectable in noncanceroustissue. Immunohistochemistry was performed directly to expression of COX-2 in tumor tissue. CONCLUSION: These results suggest that COX-2 may bean important factor for esophageal cancer and inhibition of COX-2 may be helpful for prevention and possibly treatment of this cancer.